Microdissection by Exclusion and DNA Extraction for Multiple PCR Analyses from Archival Tissue Sections
نویسندگان
چکیده
منابع مشابه
Rapid DNA extraction from ferns for PCR-based analyses.
Pteridophytes are not as well characterized at the molecular and biochemical level as some higher plants. However, to aid conservation efforts (4), there is growing interest in understanding the population biology of many fern species that are under threat. DNAbased studies offer excellent tools for such work, but are hampered in ferns by difficulties in obtaining good-quality DNA from sufficie...
متن کاملImproved resolution by mounting of tissue sections for laser microdissection.
BACKGROUND Laser microbeam microdissection has greatly facilitated the procurement of specific cell populations from tissue sections. However, the fact that a coverslip is not used means that the morphology of the tissue sections is often poor. AIMS To develop a mounting method that greatly improves the morphological quality of tissue sections for laser microbeam microdissection purposes so t...
متن کاملAn Efficient Method For DNA Extraction From Paraffin Wax Embedded Tissues For PCR Amplification Of Human And Viral DNA
Background and Objective: Formalin-fixed paraffin-embedded tissues are a valuable source of DNA for molecular studies. We designed and optimized an efficient procedure for DNA extraction from formalin-fixed paraffin embedded tissues. Materials and Methods: Seventy three blocks of cervical paraffin-embedded tissues were investigated. DNA was extracted using 45 minutes boiling in alkaline sol...
متن کاملshort note- simple and effective method for genomic dna extraction from plant and fungus for pcr-based analyses
0
متن کامل2D-PCR: a method of mapping DNA in tissue sections.
A novel approach was developed for mapping the location of target DNA in tissue sections. The method combines a high-density, multi-well plate with an innovative single-tube procedure to directly extract, amplify, and detect the DNA in parallel while maintaining the two-dimensional (2D) architecture of the tissue. A 2D map of the gene glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was created...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: BioTechniques
سال: 2000
ISSN: 0736-6205,1940-9818
DOI: 10.2144/00285bm06